Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories.
Identifieur interne : 000963 ( Main/Exploration ); précédent : 000962; suivant : 000964Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories.
Auteurs : Sibyle Etievant [France] ; Antonin Bal [France] ; Vanessa Escuret [France] ; Karen Brengel-Pesce [France] ; Maude Bouscambert [France] ; Valérie Cheynet [France] ; Laurence Generenaz [France] ; Guy Oriol [France] ; Gregory Destras [France] ; Geneviève Billaud [France] ; Laurence Josset [France] ; Emilie Frobert [France] ; Florence Morfin [France] ; Alexandre Gaymard [France]Source :
- Journal of clinical medicine [ 2077-0383 ] ; 2020.
Abstract
A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charité (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.
DOI: 10.3390/jcm9061871
PubMed: 32560044
PubMed Central: PMC7355678
Affiliations:
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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Cheynet, Valerie" sort="Cheynet, Valerie" uniqKey="Cheynet V" first="Valérie" last="Cheynet">Valérie Cheynet</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Generenaz, Laurence" sort="Generenaz, Laurence" uniqKey="Generenaz L" first="Laurence" last="Generenaz">Laurence Generenaz</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Oriol, Guy" sort="Oriol, Guy" uniqKey="Oriol G" first="Guy" last="Oriol">Guy Oriol</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Destras, Gregory" sort="Destras, Gregory" uniqKey="Destras G" first="Gregory" last="Destras">Gregory Destras</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Billaud, Genevieve" sort="Billaud, Genevieve" uniqKey="Billaud G" first="Geneviève" last="Billaud">Geneviève Billaud</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Josset, Laurence" sort="Josset, Laurence" uniqKey="Josset L" first="Laurence" last="Josset">Laurence Josset</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Frobert, Emilie" sort="Frobert, Emilie" uniqKey="Frobert E" first="Emilie" last="Frobert">Emilie Frobert</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Morfin, Florence" sort="Morfin, Florence" uniqKey="Morfin F" first="Florence" last="Morfin">Florence Morfin</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Gaymard, Alexandre" sort="Gaymard, Alexandre" uniqKey="Gaymard A" first="Alexandre" last="Gaymard">Alexandre Gaymard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32560044</idno><idno type="pmid">32560044</idno><idno type="doi">10.3390/jcm9061871</idno><idno type="pmc">PMC7355678</idno><idno type="wicri:Area/Main/Corpus">000D05</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000D05</idno><idno type="wicri:Area/Main/Curation">000D05</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000D05</idno><idno type="wicri:Area/Main/Exploration">000D05</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories.</title><author><name sortKey="Etievant, Sibyle" sort="Etievant, Sibyle" uniqKey="Etievant S" first="Sibyle" last="Etievant">Sibyle Etievant</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Bal, Antonin" sort="Bal, Antonin" uniqKey="Bal A" first="Antonin" last="Bal">Antonin Bal</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Escuret, Vanessa" sort="Escuret, Vanessa" uniqKey="Escuret V" first="Vanessa" last="Escuret">Vanessa Escuret</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Brengel Pesce, Karen" sort="Brengel Pesce, Karen" uniqKey="Brengel Pesce K" first="Karen" last="Brengel-Pesce">Karen Brengel-Pesce</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Bouscambert, Maude" sort="Bouscambert, Maude" uniqKey="Bouscambert M" first="Maude" last="Bouscambert">Maude Bouscambert</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Cheynet, Valerie" sort="Cheynet, Valerie" uniqKey="Cheynet V" first="Valérie" last="Cheynet">Valérie Cheynet</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Generenaz, Laurence" sort="Generenaz, Laurence" uniqKey="Generenaz L" first="Laurence" last="Generenaz">Laurence Generenaz</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Oriol, Guy" sort="Oriol, Guy" uniqKey="Oriol G" first="Guy" last="Oriol">Guy Oriol</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Pierre-Bénite</settlement></placeName></affiliation></author><author><name sortKey="Destras, Gregory" sort="Destras, Gregory" uniqKey="Destras G" first="Gregory" last="Destras">Gregory Destras</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Billaud, Genevieve" sort="Billaud, Genevieve" uniqKey="Billaud G" first="Geneviève" last="Billaud">Geneviève Billaud</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Josset, Laurence" sort="Josset, Laurence" uniqKey="Josset L" first="Laurence" last="Josset">Laurence Josset</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Frobert, Emilie" sort="Frobert, Emilie" uniqKey="Frobert E" first="Emilie" last="Frobert">Emilie Frobert</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Morfin, Florence" sort="Morfin, Florence" uniqKey="Morfin F" first="Florence" last="Morfin">Florence Morfin</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Gaymard, Alexandre" sort="Gaymard, Alexandre" uniqKey="Gaymard A" first="Alexandre" last="Gaymard">Alexandre Gaymard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of clinical medicine</title><idno type="ISSN">2077-0383</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charité (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32560044</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2077-0383</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>16</Day></PubDate></JournalIssue><Title>Journal of clinical medicine</Title><ISOAbbreviation>J Clin Med</ISOAbbreviation></Journal><ArticleTitle>Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1871</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/jcm9061871</ELocationID><Abstract><AbstractText>A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charité (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Etievant</LastName><ForeName>Sibyle</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bal</LastName><ForeName>Antonin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Escuret</LastName><ForeName>Vanessa</ForeName><Initials>V</Initials><Identifier Source="ORCID">0000-0002-5298-1002</Identifier><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brengel-Pesce</LastName><ForeName>Karen</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouscambert</LastName><ForeName>Maude</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0003-2841-1808</Identifier><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheynet</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Generenaz</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oriol</LastName><ForeName>Guy</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, Centre Hospitalier Lyon Sud, 69310 Pierre-Bénite, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Destras</LastName><ForeName>Gregory</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Billaud</LastName><ForeName>Geneviève</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Josset</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0002-7158-1186</Identifier><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frobert</LastName><ForeName>Emilie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morfin</LastName><ForeName>Florence</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gaymard</LastName><ForeName>Alexandre</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-8194-6126</Identifier><AffiliationInfo><Affiliation>Laboratoire de Virologie, Institut des Agents Infectieux (IAI), Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre National de Référence des Virus Respiratoires, Hospices Civils de Lyon, Groupement Hospitalier Nord, 69004 Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lyon, Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, F-69372 Lyon, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>J Clin Med</MedlineTA><NlmUniqueID>101606588</NlmUniqueID><ISSNLinking>2077-0383</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">RT-PCR</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">diagnostics</Keyword><Keyword MajorTopicYN="N">sensitivity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32560044</ArticleId><ArticleId IdType="pii">jcm9061871</ArticleId><ArticleId 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Apr;5(4):536-544</Citation><ArticleIdList><ArticleId IdType="pubmed">32123347</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li><li>Pierre-Bénite</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Etievant, Sibyle" sort="Etievant, Sibyle" uniqKey="Etievant S" first="Sibyle" last="Etievant">Sibyle Etievant</name></region><name sortKey="Bal, Antonin" sort="Bal, Antonin" uniqKey="Bal A" first="Antonin" last="Bal">Antonin Bal</name><name sortKey="Bal, Antonin" sort="Bal, Antonin" uniqKey="Bal A" first="Antonin" last="Bal">Antonin Bal</name><name sortKey="Bal, Antonin" sort="Bal, Antonin" uniqKey="Bal A" first="Antonin" last="Bal">Antonin Bal</name><name sortKey="Bal, Antonin" sort="Bal, Antonin" uniqKey="Bal A" first="Antonin" last="Bal">Antonin Bal</name><name sortKey="Billaud, Genevieve" sort="Billaud, Genevieve" uniqKey="Billaud G" first="Geneviève" last="Billaud">Geneviève Billaud</name><name sortKey="Bouscambert, Maude" sort="Bouscambert, Maude" uniqKey="Bouscambert M" first="Maude" last="Bouscambert">Maude Bouscambert</name><name sortKey="Bouscambert, Maude" sort="Bouscambert, Maude" uniqKey="Bouscambert M" first="Maude" last="Bouscambert">Maude Bouscambert</name><name sortKey="Brengel Pesce, Karen" sort="Brengel Pesce, Karen" uniqKey="Brengel Pesce K" first="Karen" last="Brengel-Pesce">Karen Brengel-Pesce</name><name sortKey="Cheynet, Valerie" sort="Cheynet, Valerie" uniqKey="Cheynet V" first="Valérie" last="Cheynet">Valérie Cheynet</name><name sortKey="Destras, Gregory" sort="Destras, Gregory" uniqKey="Destras G" first="Gregory" last="Destras">Gregory Destras</name><name sortKey="Escuret, Vanessa" sort="Escuret, Vanessa" uniqKey="Escuret V" first="Vanessa" last="Escuret">Vanessa Escuret</name><name sortKey="Escuret, Vanessa" sort="Escuret, Vanessa" uniqKey="Escuret V" first="Vanessa" last="Escuret">Vanessa Escuret</name><name sortKey="Escuret, Vanessa" sort="Escuret, Vanessa" uniqKey="Escuret V" first="Vanessa" last="Escuret">Vanessa Escuret</name><name sortKey="Etievant, Sibyle" sort="Etievant, Sibyle" uniqKey="Etievant S" first="Sibyle" last="Etievant">Sibyle Etievant</name><name sortKey="Frobert, Emilie" sort="Frobert, Emilie" uniqKey="Frobert E" first="Emilie" last="Frobert">Emilie Frobert</name><name sortKey="Frobert, Emilie" sort="Frobert, Emilie" uniqKey="Frobert E" first="Emilie" last="Frobert">Emilie Frobert</name><name sortKey="Gaymard, Alexandre" sort="Gaymard, Alexandre" uniqKey="Gaymard A" first="Alexandre" last="Gaymard">Alexandre Gaymard</name><name sortKey="Gaymard, Alexandre" sort="Gaymard, Alexandre" uniqKey="Gaymard A" first="Alexandre" last="Gaymard">Alexandre Gaymard</name><name sortKey="Gaymard, Alexandre" sort="Gaymard, Alexandre" uniqKey="Gaymard A" first="Alexandre" last="Gaymard">Alexandre Gaymard</name><name sortKey="Generenaz, Laurence" sort="Generenaz, Laurence" uniqKey="Generenaz L" first="Laurence" last="Generenaz">Laurence Generenaz</name><name sortKey="Josset, Laurence" sort="Josset, Laurence" uniqKey="Josset L" first="Laurence" last="Josset">Laurence Josset</name><name sortKey="Josset, Laurence" sort="Josset, Laurence" uniqKey="Josset L" first="Laurence" last="Josset">Laurence Josset</name><name sortKey="Morfin, Florence" sort="Morfin, Florence" uniqKey="Morfin F" first="Florence" last="Morfin">Florence Morfin</name><name sortKey="Morfin, Florence" sort="Morfin, Florence" uniqKey="Morfin F" first="Florence" last="Morfin">Florence Morfin</name><name sortKey="Oriol, Guy" sort="Oriol, Guy" uniqKey="Oriol G" first="Guy" last="Oriol">Guy Oriol</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidFranceV1/Data/Main/Exploration
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|wiki= Sante
|area= CovidFranceV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32560044
|texte= Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories.
}}
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| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
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